Assay Workflow

Assay Workflow

Genomic DNA is immediately bound to beads and subsequently fragmented and indexed allowing for earlier pooling. This streamlined workflow enables a greater sample flexibility while reducing the risk of sample mix-up and contamination. 

 

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Many of the current amplicon-based chemistries used for HLA typing require the setup of multiple long-range PCR reactions. Subsequent individual clean-up, normalization and fragmentation steps require excessive tip and plate usage.

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Following tagmentation and indexing, library fragments are cleaned up and size selected using magnetic beads. Libraries are pooled in a single tube where the regions of interest are hybridized and captured using probes. Following a final enrichment and magnetic bead cleanup, prepared libraries are quantified and denatured before loading onto an Illumina sequencing instrument.