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XM-ONE TROUBLESHOOTING

Please observe that the flowchart below is followed by explanatory text

 


 

Step 1
Important: Do not to use EDTA tubes.

 

Step 2
No obvious handling errors.

 

Step 3
Step 7 in Instructions for use.

Potential problem: Low yield of endothelial precursor cells.
Potential cause: Cells are adhering to the tube.
Solution: Use siliconized tubes according to the instructions.

Step 8 in Instructions for use.

Potential problem: Low yield of endothelial precursor cells.
Potential cause: Poor mixing during incubation with magnetic beads or too intense mixing.
Solution: Ensure that mixing is sufficient to avoid sedimentation but not so intense that the beads fail to bind to the cells.

 

Step 4
Step 10 in Instructions to use.

Potential problem: False-positive XM-One.
Potential cause: PBS not properly discarded before serum added.
Solution: Make sure that all PBS is discarded according to the Instructions for use.

 

Step 5
No obvious handling errors.

 

Step 6

Potential problem: 1. Incorrect setting in flow cytometry, often of the forward-scatter (FSC) and side-scatter (SSC). It is important to adjust the settings so that distinct cell populations can be detected. 2. Wrong cell population obtained.
Potential cause: Poor mixing during incubation with magnetic beads or too intense mixing.
Solution: Experience and handling-knowledge of flow cytometry. Different equipment types require different settings. Settings used for lymphocyte crossmatches can often be used with only minor adjustments.