TruSight HLA
TruSight HLA
CareDx (and its Olerup Subsidiary) and Illumina entered into a License and Commercialization Agreement which provides CareDx with the exclusive global distribution rights for TruSight HLA assays and the TruSight HLA Assign analysis software. CareDx & Illumina joint communication letter
As of May 25, 2018, all TruSight HLA orders should be placed with CareDx:
US (Americas): orders-us@caredx.com
Global (ex-Americas): orders-at@caredx.com
General inquiries: customercare@caredx.com
Technical support: techsupport-global@caredx.com
CareDx will work with customers to convert currently placed orders, including standing orders. CareDx and Illumina will be working together closely to seamlessly transition customers to CareDx.
TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:
- High-resolution sequencing of 11 HLA loci
- Workflow with library preparation, sequencing and data analysis/reporting
- Accurate and high-resolution HLA typing for unambiguous results
Capture Full HLA Gene Sequences
TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.
| Loci | Target Sequence |
|---|---|
| HLA-A | 4.1 kb (entire gene) |
| HLA-B | 2.8 kb (exon 1 - intron 6) |
| HLA-C | 4.2 kb (entire gene) |
| HLA-DRB1/3/4/5 | 4.6 - 5.1 kb (exon 2 - intron 4) |
| HLA-DQB1 | 6.9 kb (entire gene) |
| HLA-DPB1 | 9.7 kb (exon 2-3' UTR) |
| HLA-DQA1 | 7.3 kb (entire gene) |
| HLA-DPA1 | 10.3 kb (entire gene) |
Sample-to-Report Workflow with Optimized Data Analysis
The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexible options for post-analysis reporting of results.
High-Accuracy HLA Typing
The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.
How TruSight HLA v2 Works
TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.
Analysis Software Highlights
The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.